Fig 1: CD11b expression was highly elevated in FHR-3 treated ARPE-19 cells. (A) CD11B mRNA expression was highly elevated after 5 h and 24 h FHR-3 incubation. No expression of CD11B mRNA could be detected when cells were treated with FH. The effect of FHR-3 could be confirmed at the protein level: (B) Western blots of ARPE-19 cell lysates showed a time-dependent increase in CD11b levels (120 kDa) from 5 h – 24 h after FHR-3 incubation. Supplementary Figure 1N shows full Western blots. (C) Elevated CD11b protein levels were detected by immunofluorescence using a specific anti-CD11b antibody (red) after 5 h and 24 h of FHR-3 incubation (right panels). Scale bars 40 µm. w/o untreated control (dotted line). n.d. not detected. Mean with standard deviation is shown. *p < 0.05. (A) Wilcoxon matched-pairs signed rank test (n = 3); (B) Ordinary one-way ANOVA, Turkey’s multiple comparisons test (n = 3).